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goat anti cyclin d1 d2  (R&D Systems)


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    R&D Systems goat anti cyclin d1 d2
    Goat Anti Cyclin D1 D2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti cyclin d1 d2/product/R&D Systems
    Average 93 stars, based on 27 article reviews
    goat anti cyclin d1 d2 - by Bioz Stars, 2026-02
    93/100 stars

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    CTSV depletion attenuates growth of breast cancer cells by stalling progression through G2/M phase. (A) CTSV depletion in MCF-7 and ZR75-1 cells results in reduced cell growth when assessed by MTT assay. (B) PI staining with flow cytometry analysis identified that CTSV depletion results in fewer cells in G1, with a concomitant increase in G2/M phase. Rescue experiments were undertaken by restoring CTSV expression in sh1 cells, where cell cycle profiles were restored to that of the NTC cells. (C) Western blotting analysis of CTSV depleted cells shows that cyclin B1 expression is elevated in comparison to control cells, whereas cyclins D1/D2 and E1 remain unchanged. Tubulin expression was used as an internal loading control, with presented blots representative of at least three independent experiments. The average and standard deviation (SD) values are representative of three independent experiments, with statistical analysis was determined by two-way-ANOVA with a Tukey’s post hoc test for multiple comparisons performed using GraphPad Prism 8.

    Journal: Frontiers in Pharmacology

    Article Title: Cathepsin V regulates cell cycle progression and histone stability in the nucleus of breast cancer cells

    doi: 10.3389/fphar.2023.1271435

    Figure Lengend Snippet: CTSV depletion attenuates growth of breast cancer cells by stalling progression through G2/M phase. (A) CTSV depletion in MCF-7 and ZR75-1 cells results in reduced cell growth when assessed by MTT assay. (B) PI staining with flow cytometry analysis identified that CTSV depletion results in fewer cells in G1, with a concomitant increase in G2/M phase. Rescue experiments were undertaken by restoring CTSV expression in sh1 cells, where cell cycle profiles were restored to that of the NTC cells. (C) Western blotting analysis of CTSV depleted cells shows that cyclin B1 expression is elevated in comparison to control cells, whereas cyclins D1/D2 and E1 remain unchanged. Tubulin expression was used as an internal loading control, with presented blots representative of at least three independent experiments. The average and standard deviation (SD) values are representative of three independent experiments, with statistical analysis was determined by two-way-ANOVA with a Tukey’s post hoc test for multiple comparisons performed using GraphPad Prism 8.

    Article Snippet: The following antibodies were used in this study; goat polyclonal CTSV (BioTechne, AF1080), goat polyclonal cyclin D1/D2 (AF4196, BioTechne), mouse monoclonal cyclin E1 (MAB68101, BioTechne), rabbit monoclonal cyclin B1 (MAB60001, BioTechne), rabbit polyclonal HDAC1 (2062S, Cell Signaling), mouse monoclonal histone H1 (NBP2-45184, BioTechne), rabbit polyclonal histone H2a (NB100-56346, BioTechne), rabbit polyclonal histone H2b (NB100-56633, BioTechne), rabbit monoclonal histone H3 (4499S, Cell Signaling), rabbit monoclonal histone H4 (NBP2-80444, BioTechne), mouse monoclonal Hsc70 (MAB4148, BioTechne), mouse monoclonal Hsp90 (ab13492, Abcam), mouse monoclonal Asf1b (NBP2-61684, BioTechne), rabbit monoclonal NASP (ab181169, Abcam), mouse monoclonal GATA3 (BioTechne, MAB6330), goat polyclonal GAPDH (BioTechne, AF5718), mouse monoclonal CTSL (BioTechne, MAB9521) and rat monoclonal α-Tubulin (ab6160, Abcam).

    Techniques: MTT Assay, Staining, Flow Cytometry, Expressing, Western Blot, Comparison, Standard Deviation